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KMID : 0613820220320030232
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Journal of Life Science
2022 Volume.32 No. 3 p.232 ~ p.240
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Development of a Method for Producing Liposome Ascorbic acid with Increased Bio-absorption
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Cha Ji-Hyun
Woo Young-Min
Jo Eun-Sol
Cha Jae-Young
Lee Sang-Hyeon
Lee Keun-Woo
Kim Andre
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Abstract
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Various methods are known for preparing liposomes, the simplest being the Bangham method which has been widely used. Although it is possible to produce liposomes effectively on a small experimental level with this approach, large-scale production cannot be easily performed due to difficulties in removing the organic solvent and the size of the reactor required to form the lipid film. On the other hand, emulsion can mass produce tons of liposomes with uniform particles but has the disadvantage of a significantly low capture rate. This study therefore developed an optimal liposome processing method using heat with improved capture rate and stability, and bio-absorption experiments were performed by oral administration to SD rat alongside capture rate, particle size, and zeta potential. Through the heating method, a small and uniform liposome of about 214 nm was formed and the capture rate was 38.67%, confirming that the liposome prepared by heating has a higher capture rate than the 26.46% achieved through emulsion. Comparing blood concentrations, it showed a 1.5 to 2 fold increase in all groups, gradually decreasing until 4-12 hr. The highest blood concentration of ascorbic acid powder was about 12.017 μg/ml, the emulsion liposome 13.871 μg/ml, and the heating liposome 16.322 μg/ml, thereby showing an improved absorption rate.
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KEYWORD
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Bio-absorption rate, liposome, oral administration, particle size, zeta potentia
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